Title: Development of a sulfonamide ELISA and its comparison with LC-MS/MS method Authors
|Rubio, Fernando - ABRAXIS LLC|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 30, 2007
Publication Date: May 20, 2007
Citation: Shelver, W.L., Rubio, F.M. 2007. Development of a sulfonamide ELISA and its comparison with LC-MS/MS method. Meeting Abstract. SETAC Europe 17th Annual Meeting, May 20-24, 2007, Porto, Portugal. Technical Abstract: Sulfonamides are common chemotherapeutic agents used in veterinary and human medicines to treat bacterial and protozoa infections. Because of their widespread usage sulfonamides, such as sulfamethoxazole, are some of the most prevalent pharmaceuticals found in waterways. A sulfonamide ELISA has been developed that has a limit of detection below 20 parts per trillion for sulfamethoxazole, sulfamethoxypyridine, sulfachloropyridine, and sulfamethoxine. The within day and between day variation were below 15%. The recoveries for spiked controls (ground water and chicken muscle) were greater than 80%. No cleanup was needed for muscle samples when using phosphate buffered saline-bovine serum albumin as calibration standard diluents. A gradient was used to separate more than 15 sulfonamides in one LC-MS/MS run; however, the analysis time is greater than 40 minutes per injection. In summary, a sulfonamide ELISA has been developed that can detect multiple sulfonamides with good recovery for spiked ground water and chicken muscle samples. An LC-MS/MS sulfonamide multi-residue confirmatory analysis has been established.