Technical Abstract: Fusarium proliferatum is a major cause of maize ear rot and fumonisin contamination and also can cause wheat kernel black point disease. The primary objective of this study was to characterize nine F. proliferatum strains from wheat from Nepal for ability to cause black point and fumonisin contamination in wheat kernels. For comparative purposes, the study included three Fusarium strains from U.S. maize. Fungal strains were applied by injection, spray, or dip of spores onto spikes of five wheat cultivars. All application methods and all strains produced kernel black point, and most strains had some affect on kernel yield. Most strains produced fumonisins in kernels, but at relatively low levels (<10 'g/g, combined fumonisin B1, B2 and B3) as determined by liquid chromatography-mass spectroscopy. One strain from Nepal, however, was able to produce high levels (>100 'g/g) of fumonisins in kernels. These data indicate a potential for fumonisin contamination of wheat infected with F. proliferatum.