|Chakrabarti, Seemanti - UNIV CALIF., RIVERSIDE|
|Cardona, Carol - UNIV CALIF., DAVIS|
|Gerry, Alec - UNIV CALIF., RIVERSIDE|
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2007
Publication Date: July 14, 2007
Citation: King, D.J., Chakrabarti, S., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2007. Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Meeting Program, July 14-18, 2007, Washington, DC. p. 44. Technical Abstract: During the 2002 Exotic Newcastle Disease (END) outbreak in California arthropods were collected from two quarantined backyard poultry premises after removal of END virus infected birds. The END virus (ENDV) isolated from field collected pools of three fly species was found to have >98% homology by nucleotide sequence analysis and identical monoclonal antibody binding profiles as viruses recovered from poultry during the 2002-03 outbreak. Two fly species and beetles commonly associated with poultry operations were provided ENDV in feed and then sampled daily for up to 12 days post-infection. In the experimental flies, the virus was recovered through days 4-5 in Musca domestica and through days 7-8 in Fannia canicularis. We failed to recover virus in similar studies conducted with the Lesser Mealworm, Alphitobius diaperinus. These findings suggest biosecurity measures should include an aggressive vector control program on and near infected premises to minimize possible contact between flies from contaminated poultry premises and susceptible poultry.