Submitted to: IOBC/WPRS Bulletin (Abstract for Conference Proceedings)
Publication Type: Other
Publication Acceptance Date: September 8, 2006
Publication Date: N/A
Interpretive Summary: The saprophytic fungus, A. flavus, is air-born and infects plants through wounds. Aflatoxin contamination is well documented to be associated with wounding in corn, peanuts, cotton and tree-nuts before harvest. In general antagonists applied on crops for control of foliar diseases are exposed to environmental stress such as temperature fluctuations, water availability and UV-irradiation. A laboratory experiment was designed by using PEG (polyethylene glycol) 8000 to adjust medium aw to 0.96, which mimicked a water stress condition of –5.62 MPa.. P. anomala WRL-076 can grow at this low water activity (aw) and inhibited the growth of A. flavus.
In laboratory experiments, PEG (polyethylene glycol) 8000 was used to adjust medium aw to 0.96, which mimicked a water stress condition of –5.62 MPa.. P. anomala WRL-076 can grow at this low water activity (aw). The yeast cells formed a film and inhibited the growth of A. flavus inoculated to the medium. Two experiments were conducted in a commercial orchard in the summer of 2005. The biopesticide, P. anomala WRL-076 reduced the frequency of A. flavus colonization by 4 to 10 times and decreased the total propagules of A. flavus by 80 to 99% in comparison to nut-fruits not sprayed with the yeast.