Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 9, 2006
Publication Date: April 28, 2007
Citation: Charron, C.S., Clevidence, B.A., Britz, S.J., Novotny Dura, J. 2007. The Effect of Dose Size on Bioavailability of Acylated and Nonacylated Anthocyanins from Red Cabbage [abstract]. Experimental Biology. p. 240.7. Interpretive Summary: The Interprettive Summary is not a required entry.
Technical Abstract: Recent studies indicate that anthocyanin (ACN) intake may reverse age-related neurodegenerative declines, promote cancer protection and suppression, improve cardiovascular health, stimulate insulin secretion, and ameliorate oxidative stress. These health benefits depend on absorption and metabolic mechanisms that deliver ACNs and their bioactive metabolites to responsive tissues. We evaluated red cabbage (Brassica oleracea L. var. capitata) ACN bioavailability as reflected by urinary excretion of ACNs and ACN metabolites. Twelve volunteers consumed 100, 200, and 300 g of steamed red cabbage (containing 1.09 micromol of ACNs per g cabbage) in a crossover experimental design. Anthocyanin concentration in cabbage extract and urine was measured by HPLC with ion trap mass spectrometry. Six nonacylated and 30 acylated ACNs were detected in red cabbage, and 2 nonacylated ACNs, 8 acylated ACNs, and 5 metabolites were present in urine. Mean 24 h excretion of intact ACNs increased linearly from 45 (100-g dose) to 65 micromol (300-g dose) for acylated ACNs and 52 (100-g dose) to 79 micromol (300-g dose) for nonacylated ACNs. Urinary recovery of intact ACNs (expressed as % of ACN intake) decreased linearly from 0.041% (100-g dose) to 0.019% (300-g dose) for acylated ACNs and from 0.176% (100-g dose) to 0.085% (300-g dose) for nonacylated ACNs. The decrease in ACN recovery (%) with increasing dose size reflects the fact that excretion did not increase in the same proportion as dose size. Anthocyanin metabolites consisted of glucuronidated and methylated ACNs. Our results show that red cabbage ACNs were excreted in both intact and metabolized forms and that recovery of nonacylated ACNs in urine was over 4-fold that of acylated ACNs.