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United States Department of Agriculture

Agricultural Research Service

Research Project: BIOLOGICALLY-BASED TECHNOLOGIES FOR MANAGEMENT OF CROP INSECT PESTS IN LOCAL AND AREA-WIDE PROGRAMS

Location: Insect Behavior and Biocontrol Research Unit

Title: Molecular determinants of lepidopteran flight capacity that respond to larval diet

Authors
item Fescemyer, Howard - PENN ST UNIV, DEPT OF BIO
item Kasputis, Mara - PENN ST UNIV, DEPT OF BIO
item Macfarland, Suzanne - PENN ST UNIV, DEPT OF BIO
item Jackson, Ryan
item Meagher, Robert
item Nagoshi, Rodney
item Adamczyk, John
item Fleischer, Shelby - PENN ST UNIV, DEPT OF BIO
item Marden, James - PENN ST UNIV, DEPT OF BIO

Submitted to: National Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2006
Publication Date: December 12, 2006
Citation: Fescemyer, H.W., Kasputis, M.S., Macfarland, S.P., Jackson, R.E., Meagher Jr, R.L., Nagoshi, R.N., Adamczyk Jr, J.J., Fleischer, S., Marden, J.H. 2006. Molecular determinants of lepidopteran flight capacity that respond to larval diet. National Entomological Society of America Annual Meeting.

Technical Abstract: Alternative splicing of gene transcripts is an important mechanism by which genes give rise to phenotypic plasticity. Relative abundance of alternative splice forms of the calcium regulatory protein, troponin t, influences the performance of flight muscle. We characterized four alternative exons for troponin-t in flight muscle from Helicoverpa zea and Spodoptera frugiperda. Then, we tested the hypothesis that variation in alternative splicing of troponin t in flight muscle underlies the influence of diet on flight capacity in H. zea and S. frugiperda. Adult H. zea derived from larvae that fed on Bt, conventional corn or artificial diet. Adult S. frugiperda derived from rice or corn strain larvae fed artificial diet or starved for different lengths of time during the last instar. Flight capacity of moths was measured in terms of flight metabolic rate, flight behavior, mass, wing area, wing loading, and lipid content before quantifying their troponin t splice forms. Relative abundance of the largest alternatively spliced exons of troponin t in S. frugiperda is associated with increased flight metabolic rate, body mass and larval feeding success. Analyses of troponin t in H. zea are ongoing. These findings suggest troponin t as a molecular marker for variation in both larval nutrition and migratory capability.

Last Modified: 8/30/2014
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