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United States Department of Agriculture

Agricultural Research Service

Title: Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress

Authors
item Tattersall, Elizabeth - UNIV. OF NEVADA, RENO
item Grimplet, Jerome - UNIV. OF NEVADA, RENO
item Deluc, Laurent - UNIV. OF NEVADA, RENO
item Wheatley, Matthew - UNIV. OF NEVADA, RENO
item Vincent, Delphine - UNIV. OF NEVADA, RENO
item Osborne, Craig - UNIV. OF NEVADA, RENO
item Ergul, Ali - ANKARA UNIVERSITY
item Loman, Evan - UNIV. OF NEVADA, RENO
item Blank, Robert
item Schlauch, Karen - BOSTON UNIVERSITY
item Cushman, John - UNIV. OF NEVADA, RENO
item Cramer, Grant - UNIV. OF NEVADA, RENO

Submitted to: Functional and Integrative Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 12, 2007
Publication Date: June 1, 2007
Citation: Tattersall, E.A., Grimplet, J., Deluc, L., Wheatley, M.D., Vincent, D., Osborne, C., Ergul, A., Loman, E., Blank, R.R., Schlauch, K.A., Cushman, J.C., Cramer, G.R. 2007. Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress. Functional Integrative Genomics. 7:317-333.

Interpretive Summary: There is limited knowledge of how grapevine species will respond to the cold and saline environments of northern Nevada. We quantified gene expression and physiological changes of grapevine to osmotic and chilling stress. Concentration of chloride, phosphate, and transcripts of ABA, ethylene and jasmonate increased in response to stress. Research is continuing to determine if these stress-related changes affect wine quality.

Technical Abstract: In this study, iso-osmotic salinity (120 mM NaCl, 12mM CaCl2) and osmotic (PEG) stresses, along with chilling (5oC) stress, were applied to the cold-sensitive grapevine species V. vinifera cv. Cabernet Sauvignon. Microarray analysis of transcript abundance in shoot tips revealed that 43% of gene expression changes were solely in response to cold. Physiological responses of the plants included reduced stomatal conductance and stem water potentials as low as -0.8 MPa. Analysis of relative anion concentrations showed increases in chloride and phosphate levels in response to stress. There was a trend toward increased gene expression changes with increasing time of exposure to stress. No significant difference in gene expression were detected between salt and osmotic stress prior to the 24-hour time point. Thirty transcription factor transcripts showed a 5-fold or greater increase in abundance following stress, and seven were decreased 5-fold in abundance. Transcripts related to hormone responses were more abundant following stress, particularly for ABA, ethylene and jasmonate. Significantly fewer transcripts relating to both protein synthesis and to cell cycle/DNA processing were changed in abundance in response to cold that to either salt or PEG. Twenty transcripts corresponding to calcium signaling were increased in abundance solely in response to cold stress, while three calcium signaling transcripts were more abundant following either salt or PEG stress, implying a greater role for calcium signaling in the cold stress response.

Last Modified: 10/21/2014
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