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United States Department of Agriculture

Agricultural Research Service

Title: Bactericidal effect of several chemicals on hatching eggs inoculated with Salmonella serovar Typhimurium

Authors
item Cox, Nelson
item Richardson, Larry
item Buhr, Richard
item Musgrove, Michael
item Berrang, Mark
item Bright, W - SC STATE U

Submitted to: International Poultry Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 28, 2006
Publication Date: January 22, 2007
Citation: Cox Jr, N.A., Richardson, L.J., Buhr, R.J., Musgrove, M.T., Berrang, M.E., Bright, W. 2007. Bactericidal effect of several chemicals on hatching eggs inoculated with Salmonella serovar Typhimurium. International Poultry Forum Proceedings.P160:53.

Technical Abstract: Breeder flocks and commercial hatcheries represent an early contamination point for Salmonella entry into commercial integrated poultry operations. Utilizing effective antimicrobial treatments for hatching eggs is a critical part of reducing the incidence of Salmonella colonized chicks on the farm. The objective of this study was to evaluate the bactericidal effect of several chemicals on Salmonella contaminated hatching eggs. Four replications (n=10/treatment) were conducted to determine the efficacy of seven commercially available compounds. The compounds tested were A) hydrogen peroxide B) water/oil emulsion droplets stabilized by detergent C) peroxyacetic acid D) four quaternary ammonium compounds attached to a polymer E) two quaternary ammonium compounds, one biquanide compound and bronopol attached to a polymer F) N-alkyl dimethyl benzyl ammonium chloride and stabilized urea and G) polyhexamethylenebiquanide hydrochloride (PHMB). A naladixic acid resistant Salmonella serovar Typhimurium was inoculated (104 cfu/ml) onto fertile hatching eggs by drip inoculation. Controls included 1) a positive control (no spray application) and 2) water control (spray containing water to take into account rinsing effects). The E and G compounds had a 100% reduction and both of these chemicals included a biquanide. The D and C compounds were also effective with a 95% and 93.5% reduction, respectively. The B and F compounds were the least effective of all chemicals with a reduction of 40% and 47.5% respectively. Hydrogen peroxide, which has been used by the poultry industry, had a 70% reduction and the water control produced a 10% reduction. Several antimicrobials tested were more effective than hydrogen peroxide. More detailed studies will be required to adequately evaluate these antimicrobials.

Last Modified: 4/20/2014