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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC MECHANISMS AND MOLECULAR GENETIC RESOURCES FOR MAIZE

Location: Plant Genetics Research

Title: Physical and Genetic Structure of the Maize Genome Reflects its Complex Evolutionary History

Authors
item Wei, Fusheng - UNIV OF ARIZONA-TUCSON
item Coe Jr, Edward - USDA-RETIRED
item Nelson, William - UNIV OF ARIZONA-TUCSON
item Engler, Fred - UNIV OF ARIZONA-TUCSON
item Bharti, Arvind - RUTGERS STATE UNIV OF NJ
item Butler, Ed - UNIV OF ARIZONA-TUCSON
item Kim, Hyeran - UNIV OF ARIZONA-TUCSON
item Goicoechea, Jose Luis - UNIV OF ARIZONA-TUCSON
item Lee, Seunghee - UNIV OF ARIZONA-TUCSON
item Fuks, Galina - RUTGERS STATE UNIV OF NJ
item Sanchez-Villeda, Hector - UNIVERSITY OF MISSOURI
item Schroeder, Steven - UNIVERSITY OF MISSOURI
item Fang, Zhiwei - UNIVERSITY OF MISSOURI
item McMullen, Michael
item Davis, Georgia - UNIVERSITY OF MISSOURI
item Bowers, John - UNIV OF GEORGIA-ATHENS
item Paterson, Andrew - UNIV OF GEORGIA-ATHENS
item Schaeffer, Mary
item Gardiner, Jack - UNIVERSITY OF MISSOURI
item Cone, Karen - UNIVERSITY OF MISSOURI
item Messing, Joachim - RUTGERS STATE UNIV OF NJ
item Soderlund, Carol - UNIV OF ARIZONA-TUCSON
item Wing, Rod - UNIV OF ARIZONA-TUCSON

Submitted to: PLoS Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 5, 2007
Publication Date: July 20, 2007
Citation: Wei, F., Coe Jr, E., Nelson, W., Engler, F., Bharti, A.K., Butler, E., Kim, H., Goicoechea, J., Lee, S., Fuks, G., Sanchez-Villeda, H., Schroeder, S., Fang, Z., Mcmullen, M.D., Davis, G., Bowers, J.E., Paterson, A.H., Schaeffer, M.L., Gardiner, J., Cone, K., Messing, J., Soderlund, C., Wing, R.A. 2007. Physical and Genetic Structure of the Maize Genome Reflects its Complex Evolutionary History. PLoS Genetics. 3(7):e123.

Interpretive Summary: Corn (maize) is the most important crop in the United States and one of the most important plant model species for genetic and biological research. An essential resource to move forward with maize research is a complete genome sequence. One requirement for obtaining a complete sequence is the availability of an integrated genetic and physical map. In this manuscript we report on the development of a sequence ready, fingerprinted contig physical map that covers 93.5% of the maize genome. Analysis of the gene positions in the maize physical map relative to that of the rice genome provides evidence for both a recent and ancient polyploidy origin for the maize genome. These results are important to the maize research community as the physical map is the foundation of the current maize whole genome sequencing effort.

Technical Abstract: Maize (Zea mays L.) is one of the most important cereal crops and a model for the study of genetics, evolution, and domestication. To better understand maize genome organization and build a framework for genome sequencing, we constructed a sequence ready fingerprinted contig (FPC)-based physical map that covers 93.5% of the genome, of which 86.1% is aligned to the genetic map. The FPC map contains 23,954 gene-containing sequences that enabled us to align nearly 73% of the anchored maize genome to the rice genome. In collinear regions, 1 kb in rice corresponds to an average of 3.2 kb in maize, with the overall 6.2-fold genome size expansion in maize explained by most regions in rice corresponding to two regions of maize resulting from its recent polyploid origin. Inversions account for the majority of chromosome structural variations during subsequent maize diploidization. We also find clear evidence of ancient genome duplication predating the divergence of the progenitors of maize and rice. Reconstructing the paleoethnobotany of the maize genome indicates that the progenitors of modern maize contained 10 chromosomes.

Last Modified: 4/19/2014
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