ARS | Publication request: COMPARATIVE PROTEOMIC ANALYSIS OF STEM TISSUE AND XYLEM SAP FROM PIERCE'S DISEASE RESISTANT AND SUSCEPTIBLE GRAPEVINES

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: September 25, 2006
Publication Date: November 27, 2006
Citation: Lin, H., Fritschi, F.B., Yang, L., Walker, A. 2006. Comparative proteomic analysis of stem tissue and xylem sap from pierce's disease resistant and susceptible grapevines. Proceedings of CDFA Pierce's Disease Control Program Research Symposium, November 27-29, 2006. p.294-297.

Interpretive Summary: Analyses of host plant resistance and susceptibility mechanisms to Xylella fastidiosa (Xf) infection are critical for management of Pierce’s Disease (PD) of grape and other crops. Protein analyses of stem tissue and xylem sap samples were initiated to elucidate mechanisms of disease resistance and susceptibility. Samples from one highly resistant and two susceptible grape lines were collected at multiple time points following inoculation with Xf and compared to each other and with control samples from uninoculated plants. Protein profiles, observed as a result of two-dimensional gel electrophoresis, indicate that differential expression of numerous proteins are dependent upon both plant line and infection status. Differentially expressed proteins identified by mass spectroscopy comprised a wide range of functional classes. The importance of these proteins with respect to host-pathogen interactions leading to PD resistance or susceptibility will be established in future studies.

Technical Abstract: Analyses of host plant resistance / susceptibility mechanisms to Xylella fastidiosa (Xf) infection are critical for understanding host-pathogen interactions. Proteomic analyses of stem tissue and xylem sap samples were initiated to complement genomic approaches employed in elucidating Pierce’s Disease resistance mechanisms. Samples from one highly resistant and two susceptible grape genotypes were collected at multiple time points post-inoculation from control and Xf-inoculated plants. Two-dimensional gel electrophoresis revealed numerous proteins that were differentially expressed and dependent on plant genotype and/or inoculation treatment. Proteins identified by oMALDI-TOF comprised a wide range of functional types. The importance of these proteins with respect to host-pathogen interactions will be investigated further.