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United States Department of Agriculture

Agricultural Research Service

Title: The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus (CCLV) and its Genome Organization

Authors
item Guerra-Moreno, A. - UNIV OF FL, CREC, LAKE AL
item Keremane, Manjunath
item Brlansky, R. - UNIV OF FL, CREC, LAKE AL
item Lee, Richard

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 2007
Publication Date: July 31, 2007
Citation: Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus, (CCLV) and its Genome Organization. Phytopathology 97: S 174

Technical Abstract: Leprosis, an emerging invasive disease of citrus in Central America, has been present in South America for several years. Leprosis does not occur in the U.S. or the Caribbean Islands, but these regions are threatened by the disease. We have described the association of a bipartite, positive-sense RNA virus associated with the cytoplasmic citrus leprosis. The CCLV RNAs were identified from a cDNA library created from citrus naturally infected with the cytoplasmic leprosis disease from Panama. Here we report the complete sequence of the CCLV bipartite genome. RNA 1 possesses two open reading frames (ORFs). ORF 1 in RNA 1 encodes a putative 276 kDa polyprotein containing domains similar to the Sindbis-like virus super group having putative methyltransferase, cysteine protease, RNA helicase and RNA dependent RNA polymerase motifs. ORF 2 in RNA 1 shows no similarity with other sequences in the Genbank. RNA 2 has three ORFs. While ORFs 1 and 3 of RNA 2 show no similarity with sequences in the Genbank, ORF 2 encodes a putative 30.6 kDa viral movement protein.

Last Modified: 7/23/2014