ARS | Publication request: Identification and Mapping of Drought Responsive Genes in Cotton

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2006
Publication Date: November 17, 2006
Citation: Payton, P.R., Kebede, H., Allen, R., Wright, R. 2006. Identification and Mapping of Drought Responsive Genes in Cotton[abstract]. International Center for Arid & Semi-Arid Land Studies Conference. Lubbock, Texas. November 15-17, 2006.

Technical Abstract: We have developed and sequenced a sizable EST library from tetraploid cotton (G. hirsutum) under drought-stress and well-watered conditions. This collections of ESTs is serving as a contribution to a significant collection currently used as the standard tool for expression profiling in cotton. The principal aim of this study was to investigate the molecular mechanisms underlying the response to water-deficit stress in cotton plants and developing cotton fibers by merging expression analysis, physiological characters, and genetic mapping of candidate drought responsive genes. In silico analyses of ESTs generated from drought-stressed and irrigated cotton boll and tissue libraries and gene expression analysis of stressed tissues were used to identify candidate genes (ESTs) for controlling responses underlying stress response in cotton. The association of these candidate genes with QTLs that influence plant productivity, physiological traits, and fiber quality in response to drought stress conditions was investigated and will be presented at this meeting.