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United States Department of Agriculture

Agricultural Research Service

Title: Analysis of manure-derived oxytetracycline in amended soil

Authors
item Wang, Qiquan - UNIVERSITY OF CALIFORNIA
item Yates, Scott

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 10, 2006
Publication Date: September 14, 2006
Citation: Wang, Q., Yates, S.R. 2006. Analysis of manure-derived oxytetracycline in amended soil. American Chemical Society National Meeting. pp# AGRO-5

Technical Abstract: As a member of tetracycline antibiotic family, oxytetracycline is widely administered to animals. With the application of manure from medicated animals as fertilizer into agricultural land, oxytetracycline may enter the environment. For studying oxytetracycline contamination and its fate in agricultural soil, an analytical method was developed to analyze oxytetracycline in manure amended soils. Sixteen grams of manure-amended soil was extracted with 2.5 g citric acid, 1.5 oxalic acid, and 15 mL methanol:water = 90:10 solution and extraction was repeated for a total of 3 times. Extract was directly analyzed with high performance liquid chromatography. The extraction recovery of the developed method showed to be much higher than those with 2% HCl methanol or pH = 4 EDTA-McIlvaine buffer. Effect of manure amended ratio, manure type, and soil type on extraction recovery was investigated. Detection limit, linearity, and specificity of the developed method were also studied

Last Modified: 9/2/2014
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