Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 24, 2006
Publication Date: September 1, 2006
Citation: Wolf, W.R., Goldschmidt, R.J. 2006. Measurement of Niacin in a Variety of Food Samples by High Performance Liquid Chromatography-Stable Isotope Dilution Mass Spectrometry. AOAC Annual Meeting, September 15, 2007, Minneapolis, Minnesota. Technical Abstract: The availability of deuterium-labeled nicotinic acid makes stable isotope dilution mass spectrometry (SIDMS) coupled with liquid chromatography (LC) an attractive option for the determination of the water-soluble B-vitamin, niacin, in food samples. We have developed a method based on AOAC Peer-Verified Method PVM1:2000, which includes acid digestion, solid phase extraction (SPE) with a strong cation exchange (SCX) column, and reversed phase chromatography with a C18 column. Ionization is by positive ion electrospray. Although analysis can be accomplished in selected ion recording (SIR) mode, it is subject to interference problems similar to those found with other LC determinations of niacin. The additional selectivity of multiple reaction monitoring (MRM) mode, however, largely eliminates interference problems. The method is applied to a variety of food samples, both fortified and those with only endogenous niacin, including some with sub-ppm niacin levels. The method is capable of determining niacin levels even to the ppb range.