|Chang, In-Ae - KYUNGPOOK NATL UNIV KOREA|
|Kim, In-Hwan - KOREA UNIV, KOREA|
|Kang, Sun-Chul - DAEGU UNIV, KOREA|
|Kim, Hak-Ryul - KYUNGPOOK NATL UNIV KOREA|
Submitted to: Applied Microbiology and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 4, 2006
Publication Date: January 15, 2007
Citation: Chang, I., Kim, I., Kang, S., Hou, C.T., Kim, H. 2007. Production of 7, 10-dihydroxy-8(E)-octadecenoic acid from triolein via lipase induction by Pseudomonas aeruginosa PR3. Applied Microbiology and Biotechnology. 74:301-306. Interpretive Summary: Previously, we discovered a new microbial strain PR3 that produced 7,10-dihydrox-fatty acid from oleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, and coatings materials. Although strain PR3 can convert oleic acid to value-added hydroxyl fatty acids, it will be nice if the strain PR3 can convert vegetable oil directly. Now we found that strain PR3 possesses lipase activity and converts directly from triolein to oleic acid and then to dihydroxy fatty acid product. These findings improve the possibility of converting soybean oil directly to value-added hydroxyl fatty acid product.
Technical Abstract: Hydroxy fatty acids have gained important attentions because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The new bacterial isolate Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Up to now, the substrates used for microbial HFA production were free fatty acids. However, triacylglyceride, specifically triolein is possible to be utilized as a substrate by microbial enzyme system involved in HFA production from oleic acid. In this study we used triolein as a substrate and firstly report that triolein could be efficiently utilized by PR3 to produce DOD. Triolein was first hydrolyzed into oleic acid by the triolein-induced lipase and then the released oleic acid was converted to DOD by PR3. Results from this study demonstrated that natural vegetable oils, without being intentionally hydrolyzed, could be used as efficient substrate for the microbial production of value-added hydroxy fatty acids.