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Submitted to: Molecular Biosystems
Publication Type: Review Article Publication Acceptance Date: 9/21/2006 Publication Date: 9/21/2006 Citation: Lee, J., Cooper, B. 2006. Alternative workflows to replace 2d-page for the construction of plant proteome maps. Molecular Biosystems. 2:621-626. Interpretive Summary: This review article is aimed toward plant scientists who are interested in knowing their options for choosing different technologies that will allow them to identify plant proteins. Options include isolating proteins from plant cells by fractionating a cell into smaller components, using different detergents to liberate proteins from cell fractions and applying various techniques to separate one protein from another. By combining different sample procesing and proteins separation strategies, researchers will be able to identify more proteins from plant cells and thus define the total protein composition of the cell. Such knowledge is directly applicable to knowing wheteher there are sufficient amounts of protein in feed and food and whether cells have the necessary protein to fight disease. This knowledge will be useful to plant and cell biologists. Technical Abstract: High-throughput separations are intrinsic to the subsequent detection and analysis of peptides and proteins by mass spectrometry. Combined, efficient separation and detection methods lead to the analysis of thousands of proteins within a sample, cell or tissue and contribute to the eventual construction of proteome maps that can be used to define a cell type or cellular state. Whereas researchers have had past success in using 2D gels to separate proteins for subsequent mass spectrometry analysis, better separation and, consequently better results could be obtained if they adapted HPLC separations or other techniques that can be used to improve throughput. This review is aimed toward plant scientists who have special separation needs due to the nature of plant cells and who could benefit from knowing the technological options and requirements for adopting them. By combining different sample processing and protein separation strategies, researchers will be able to improve the quality of their proteomic reference maps and gain new information about the proteins that define plant cells. |
