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United States Department of Agriculture

Agricultural Research Service

Title: A Cost-Effective High Throughput Genotyping Methods

Authors
item Brady, Lee
item Anderson, James - UNIV. OF MN
item Smith, Kevin - UNIV. OF MN
item Chao, Shiaoman

Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2005
Publication Date: December 10, 2005
Citation: Brady, L.M., Anderson, J., Smith, K., Chao, S. 2005. A cost-effective high throughput genotyping methods, page 18. National Fusarium Head Blight Forum Proceedings, Dec 11-13, 2005, Milwaukee, WI.

Technical Abstract: Although extensive breeding effort has been directed at improving resistance to Fusarium head blight (FHB) in wheat and barley throughout the region, the rate of releasing resistant cultivars is slow. The main challenges lie in the complex inheritance of FHB resistance, and screening of a large number of breeding lines by conventional phenotypic testing. DNA markers have been identified and tagged to a few major resistance genes in both wheat and barley. But widespread application of marker assisted selection in wheat and barley breeding for FHB resistance has been limited up until now. The recent rapid advancement of high throughput platforms and DNA-based diagnostic assay technologies have enabled the Fargo genotyping lab, along with the wheat and barley breeders from Minnesota, to initiate a pilot study to establish a cost-effective working protocol that is amenable to automation. This protocol has been implemented in the breeding programs to enhance wheat and barley breeding efforts in selecting and releasing lines resistant to FHB. A detailed method from sample preparation by the breeders to genotyping data delivery from the Fargo genotyping lab will be presented.

Last Modified: 10/22/2014
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