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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #198837

Title: How Many Viruses Are Involved In Rubus Mosaic Disease? Three Novel Viruses Isolated From Raspberry

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Panhellenic Phytopathogical Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2006
Publication Date: 10/16/2006
Citation: Tzanetakis, I.E., Martin, R.R. 2006. How many viruses are involved in Rubus mosaic disease? Three novel viruses isolated from raspberry . Panhellenic Phytopathogical Conference.

Interpretive Summary:

Technical Abstract: Abstract in Spanish, Translation by R.H. Converse. One raspberry accession that originated in Scotland and was maintained in an insect-free screenhouse caused severe symptoms including leaf mottling, epinasty and apical necrosis when grafted onto black raspberry (R. occidentalis) cv. 'Munger' indicators. The symptoms classified the plant as a Rubus mosaic disease positive. Rubus mosaic disease was first recognized in the 1920's and believed to be caused by various combinations of four or more viruses some of which are still understudied in the molecular level. Double-stranded RNA (dsRNA) was extracted and cloned, disclosing the presence of three novel viruses. Phylogenetic analysis indicated that the first virus clusters with the Closterovirus genus, which represents the aphid-transmitted members of the Closteroviridae family. Based on the highly conserved HSP70h and RdRp genes it is most closely related to Citrus tristeza virus. The second virus is related to several members of the Flexiviridae that infect rosaceous hosts (Cherry green ring mottle virus and Cherry necrotic rusty mottle virus). The third virus is related to a group of insect infecting picorna-like viruses, similar to the recently identified Strawberry latent virus. Detection protocols have been developed for all three viruses. The closterovirus is the most widespread of all three and the complete nucleotide sequence of the virus has been acquired. We are presently working with scientists at SCRI, Scotland to determine if the closterovirus is one of the previously identified virus-like agents in Rubus.