Location: Aquatic Animal Health Research
Title: MOLECULAR CLONING AND EXPRESSION OF CHANNEL CATFISH, ICTALURUS PUNCTATUS, COMPLEMENT MEMBRANE ATTACK COMPLEX INHIBITOR CD59 Authors
Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: August 10, 2006
Publication Date: August 22, 2006
Citation: Yeh, H., Klesius, P.H. 2006. Molecular cloning and expression of channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59. Genbank. Technical Abstract: The channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59 gene was cloned and analyzed. Total RNA from tissues was isolated and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the CD59 transcript. The complete channel catfish CD59 cDNA comprised 1109 bp including a 132-bp 5’-untranslated, a 360-bp open reading frame and a 617-bp 3’-untranslated regions. The open reading frame encodes a putative protein of 119 amino acid residues with calculated molecular mass (without potential glycosylation) of 13.2 kDa.