|Liu, Zhenyu - UNIV OF WISC - MADISON|
Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 17, 2006
Publication Date: July 24, 2006
Citation: Halterman, D.A., Liu, Z. 2006. Proteomic analysis of potato late blight resistance mediated by the RB resistance gene. Potato Association of America Proceedings. 90th Annual Meeting, July 24-27, 2006, Madison, Wisconsin. p. 17. Technical Abstract: Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A gene RB, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers robust resistance to potato late blight. RB encodes a protein belonging to the CC-NBS-LRR (coiled coil-nucleotide binding site-leu-rich repeat) class of resistance proteins. It is likely involved in perception of Phytophthora and signaling to elicit resistance within the plant cell cytoplasm. We are using proteomics to understand the disease resistance mechanism mediated by RB. Complex protein mixtures are first separated by two-dimensional electrophoresis (2DE) according to charge and size (molecular weight). Selected proteins resolved by 2DE are then identified by mass spectrometry (MS). In these experiments, we compared proteins isolated from a late blight resistant transgenic potato plant SP951, containing a single copy of RB, with those from the susceptible control Katadhin (Solanum tuberosum cv. Katadhin) at 0, 24, and 72h post-inoculation using 2DE followed by MS.