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United States Department of Agriculture

Agricultural Research Service

Title: Identification of Genes Involved in Cold Resistance During Seed Germination and Postgermination of Rice Using Comparative Proteomics and Genomics

item Yuan, Joshua - UNIV TENN, KNOXVILLE
item Gealy, David
item Stoming, Terry - PROTN DISC,PISCATAWAY,NJ
item Stewart JR., C Neal - DEPT PLANT SCI,UNIV TENN

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 2006
Publication Date: July 1, 2006
Citation: Yuan, J.S., Gealy, D.R., Stoming, T., Stewart Jr., C. 2006. Identification of genes involved in cold resistance during seed germination and postgermination of rice using comparative proteomics and genomics [abstract]. Proceedings American Society of Plant Biologists Annual Meeting. Paper No. P09042.

Technical Abstract: Understanding the molecular mechanisms underlying the low temperature germinability is important in expanding the niche of cereal crops. US red rice accessions provide an excellent source of new alleles for improving cultivated rice yield, quality, stress tolerance, and disease resistance. Here we present comparative proteomics and genomics data identifying gene candidates involved in low temperature germinability that may be useful in crop improvement. US red rice accessions exhibit a diverse range of phenotypes in low temperature germinability and postgermination cold tolerance. Two red rice accessions were selected for further genomics and proteomics studies because of their extreme phenotypes. US red rice accession 16B was chosen as a cold resistant line and accession 18A was chosen as a cold sensitive line. 2D-DIGE technique was employed to profile the differential protein expression in the embryo of 16B vs. 18A after two hours of cold treatment. The 2D-DIGE images were analyzed for protein differential expression and candidate proteins are identified by MALDI-TOF MS for peptide fingerprinting. Forty seven percent of the spots can be identified with candidate proteins, among which are an NAD/NADP-dependent oxidoreductase, a heat shock protein, an ABA induced protein, and others. Microarray experiments were also carried out to compare the gene expression pattern of 16B,18A and the Nipponbare control after cold treatment. Many genes regulated by GA or ABA pathways were found to be differentially expressed among the different red rice accessions after cold treatment. In combination, genomics and proteomics approaches rendered a group of gene- and protein-candidates that might be involved in cold tolerance in the germination and postgermination developmental stages.

Last Modified: 4/20/2015