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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #198533

Title: Effect of Growing Environments on Sugars and Acid Developmet in transgenic Raspberry bushy dwarf virus resistant 'Meeker' red raspberry

Author
item MAHLER, SARAH - OREGON STATE UNIVERSITY
item QIAN, MICHAEL - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Institute of Food Technologies
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/2006
Publication Date: 8/12/2006
Citation: Mahler, S., Qian, M., Martin, R.R. 2006. Effect of growing environments on sugars and acid developmet in transgenic Raspberry bushy dwarf virus resistant 'Meeker' red raspberry. Institute of Food Technologies.

Interpretive Summary:

Technical Abstract: Raspberry Bushy Dwarf Virus (RBDV) causes significant reduction in yield and crumbly fruit in raspberries as well as raspberry-blackberry hybrids (such as Marion). There is currently no effective treatment once a plant is infected and the only effective prevention is using resistant varieties. Genetic modifications were made to ‘Meeker’ red raspberries in order to impart resistance to RBDV while maintaining the characteristics of ‘Meeker’ flavor, including the sweetness, acidity and aroma. After the challenging study, five RBDV-resistant transgenic lines were selected and grown in Oregon and Washington for fruit quality evaluation. Fruit quality parameters such as °Brix, titrable acidity, sugar and organic acid profiles were evaluated on wild type and five transgenic ‘Meeker’ raspberries grown in Oregon and Washington for the years 2004 and 2005. Significant differences (p-values<0.05) were found between years for all tests performed. In addition, °Brix and titratable acidity were significantly affected by location with berries grown in Washington having higher acid and lower °Brix than Oregon. Although fruit quality is affected by season and growing environment, the five transgenic lines behaved similarly to the wild type ‘Meeker’ red raspberry. Statistical analysis demonstrated that all five RBDV-resistant transgenic lines were not different from the wild type “Meeker’ red raspberry. Although the true-to-type lines need to be tested for other characteristics, the results in this study suggested the possibility of the transgenic RBDV-resistant lines as a replacement for the wild type ‘Meeker’ red raspberry.