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United States Department of Agriculture

Agricultural Research Service

Research Project: ENVIRONMENTAL AND GENETIC FACTORS AFFECTING PATHOGEN PERSISTENCE IN ANIMAL WASTE AND TRANSFER TO CROPS Title: Electrolytic Disinfection of Escherichia Coli and Coliform Bacteria in a Batch Cell with Dsa Electrodes

Author
item HERNLEM, BRADLEY

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 20, 2006
Publication Date: May 20, 2006
Citation: Hernlem, B.J. 2006. Electrolytic disinfection of escherichia coli and coliform bacteria in a batch cell with dsa electrodes [Abstract]. ISAC XXIII International Congress. Abstract #229; Poster # P83.

Technical Abstract: Electrolytic treatment of dairy manure lagoon water using DSA electrodes is shown to produce a progressive disinfection of native coliforms and introduced E. coli. The disinfectant effect continues post-treatment for several minutes. To further examine the process, flow cytometry was employed to study the disinfection of E. coli suspended in salt solutions. Bacterial viability was estimated both by plate counting and by the use of membrane integrity “viability” staining. The two methods displayed proportional agreement. Furthermore, it was observed that electrolytic treatment resulted in not only a decrease in the proportion of putatively “live” cells but also in a reduction of overall bacterial concentration, i.e. there appears to be a physical loss of bacteria as well as degradation in cell viability. Plate counting alone is not sufficient to show this effect.

Last Modified: 9/29/2014