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Title: INVESTIGATION OF INNATE IMMUNE RESPONSE TO THREE DIFFERENT EIMERIA SPECIES USING CHICKEN CDNA MACROPHAGE MICROARRAY

Author
item DALLOUL, RAMI - VISITING SY
item BLISS, TRAVIS - UNIV. DELAWARE
item PARK, DONG - VISITING SY
item KEELER, CALVIN - UNIV. DELAWARE
item Lillehoj, Hyun

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/28/2006
Publication Date: 10/21/2008
Citation: Dalloul, R.A., Bliss, T.W., Hong, Y.H., Park, D.W., Keeler, C.L., Lillehoj, H.S. 2008. Investigation of innate immune response to three different eimeria species using chicken cdna macrophage microarray. International Research Group Meeting. Oct 21-24. Paris, France.

Interpretive Summary:

Technical Abstract: Coccidiosis is recognized as the major parasitic disease of poultry and is caused by the apicomplexan protozoa Eimeria. Increasing evidence shows the complexity of the host immune response to Eimeria and microarray technology presents a powerful tool for the study of such an intricate biological process. Using an avian macrophage microarray containing 4,906 unique gene elements, we identified important host genes whose expression changed following infection of macrophages with sporozoites of E. tenella, E. acervulina, and E. maxima. This approach enabled us to identify a common core of 25 genetic elements whose transcriptional expression is induced or repressed by exposure to Eimeria sporozoites and to identify additional transcription patterns unique to each individual Eimeria species. Besides inducing the expression of IL-1 beta, IL-6, and IL-18 and repressing the expression of IL-16, Eimeria treated macrophages were commonly found to induce the expression of the CCL chemokine family members MIP-1 beta (CCLi1), K203 (CCLi3), and ah221 (CCLi7). However, the CXCL chemokine K60 (CXCLi1) was found to be induced by macrophage exposure to E. tenella but was repressed upon macrophage exposure to E. maxima and E. acervulina. Fundamental analysis of avian chemokine and cytokine expression patterns offers insight into the unique avian immunological responses to these related but biologically unique pathogens.