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United States Department of Agriculture

Agricultural Research Service

Title: Sensitivity of Serological Assays in Detection of Cucurbit Yellow Stunting Disorder Virus

Authors
item Abou-Jawdah, S. Eidi - UNIV OF BEIRUT, LEBANON
item Atamian, H - UNIV OF BEIRUT, LEBANON
item Sobh, H - UNIV OF BEIRUT, LEBANON
item Havey, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2006
Publication Date: June 22, 2006
Citation: Abou-Jawdah, S., Atamian, H., Sobh, H., Havey, M.J. 2006. Sensitivity of serological assays in detection of cucurbit yellow stunting disorder virus [abstract]. Proceedings of the 12th Mediterranean Phytopathological Congress. p. 114

Technical Abstract: Cucurbit yellow stunting disorder virus (CYSDV), transmitted by the whitefly Bemisia tabaci, is a major threat to cucurbit production in the Mediterranean region and has been reported in other geographic regions. Detection of CYSDV is mainly based on nucleic acid based methods. Recently antibodies produced against the recombinant coat protein were produced. The present work evaluates the sensitivity of three serological assays for detection and for relative quantification of CYSDV concentration in infected tissue. Tissue blot immunoassay is a reliable, fast and sensitive method that allows detection of CYSDV within 5-6 days post- inoculation (PI). ELISA is less sensitive, it detects CYSDV 8-9 days PI, Dot blot immunoassay also detects the virus 8-9 days PI. Serial dilutions of infected extracts showed that DBIA is at least 10 times more sensitive than ELISA. Both ELISA and DBIA can be used in quantitative assays, with a linear range of detection between 32-64X. Therefore, for surveys and preliminary variety trial tests, serological assays may present an inexpensive alternative to nucleic acid based methods for the detection and relative quantification of CYSDV.

Last Modified: 12/21/2014
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