|Vagnozzi, Ariel - OAKRIDGE INST FOR SCIENCE|
|Knowles, Nick - INST ANIMAL HEALTH, UK|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2006
Publication Date: November 27, 2006
Citation: Hollister, J.R., Vagnozzi, A., Knowles, N.J., Rieder, A.E. 2006. Molecular Analysis of Bovine Rhinovirus Type 2 Shows a Close Relationship to the Aphthoviruses. European Study Group on the Molecular Biology of Picornaviruses (EUROPIC) 2006 Meeting, page A5. Technical Abstract: Bovine rhinovirus 2 (BRV-2) the causative agent of respiratory disease in cattle is currently an unclassified species tentatively assigned to the genus Rhinovirus in the family Picornaviridae. However, previous analysis of the sequence from partial amplicons in the 3D and P1 regions of the genome suggest that BRV-2 is more closely related to foot-and-mouth disease virus (FMDV) (Knowles, Europic 2005). In the current study we have extended those observations by isolating an almost full-length cDNA encoding the genome of BRV-2 and performing bioinformatics analysis on the sequence. In agreement with the previous data, our study shows that BRV-2 is highly related to FMDV and possesses a putative Leader protease and a poly(C) tract. To generate preliminary alignments for comparison of the BRV-2 polyprotein with that of FMDV, a consensus sequence was generated for each of the seven FMDV serotypes and then aligned with the BRV-2 sequence. Examination of the amino acids immediately flanking the predicted cleavage sites within the polyprotein revealed conservation between BRV-2 and FMDV in most cases. A putative VPg encoded by BRV-2 is also more similar to the VPg’s found in FMDV serotypes than those of rhinoviruses. Moreover, unlike FMDV that encodes three VPg’s, BRV-2 encodes only one. A more global comparison of the BRV-2 genome with the FMDV suggests that as much as 40% of the amino acid residues in the P1 region, excluding VP1, are identical. Moreover, preliminary alignments indicate that amino acid residues in 2A, 2C, 3C and 3D are as much as 70%, 50%, 48%, and 63% identical, respectively. Isolation of the remainder of the BRV-2 genome, upstream of the poly(C), and further analysis including BRV-1 and BRV-3 is currently underway.