ARS | Publication request: EFFECT OF POLLINATOR ON HAPLOID INDUCTION IN POTATO

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 30, 2006
Publication Date: July 28, 2006
Citation: Jansky, S.H. 2006. Effect of pollinator on haploid induction in potato [abstract]. Proceedings of the 100th Annual Meeting of the Botanical Society of America. Paper No. 529.

Technical Abstract: The wild germplasm resources of potato are rich in genetic diversity and are readily available through the U.S. Potato Genebank. While the cultivated potato is tetraploid (2n=4x), most wild Solanum species are diploid (2n=2x). However, valuable genes from many of these species can be readily introgressed into haploids (2n=2x) of the cultivated potato. Parthenogenetic haploids can be produced through crosses to specific diploid pollinators. Several pollinators have been identified in S. tuberosum Phureja Group. All contain an embryo spot marker to distinguish between haploids and hybrids. Crosses were made between tetraploid potato clones and six haploid-inducing pollinators. The pollinators varied in their ability to extract haploids, as indicated by differences in the number of haploids produced per pollination. In addition, the proportion of hybrid versus haploid offspring varied among pollinators. The characterization of differences among pollinators will help to both improve breeding efficiency and elucidate the mechanism(s) by which haploids are generated.