|Schultz, Sarah - UNIVERSITY OF ILLINOIS|
Submitted to: American Society of Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 14, 2006
Publication Date: November 13, 2006
Citation: Schultz, S.J., Nelson, R.L. 2006. Seed protein and oil qtl mapping in three backcross soybean populations. In 2006 Agronomy Abstracts. ASA, Madison, WI.[CD-ROM computer file] Technical Abstract: Soybean seed has the highest concentration of crude protein among all sources of vegetable protein. Currently, soybean meal accounts for two-thirds of the total value seed. The objectives of this study were to identify QTL associated with protein and oil in three backcross populations and to assess genetic variability among high protein lines based on identified QTL. Three backcross populations were used: Williams 82 (2) x PI 407788A (pop 407), Williams 82 (2) x PI 423948A (pop 423), and Williams 82 (2) x PI 437088A (pop 437). All populations were grown in 2005 with two replications at three locations in east central Illinois. Pop 407 had 125 BC1F5:7 lines, pop 423 had 129 BC1F5:7 lines, pop 437 had 165 BC1F5:7 lines and all populations included parental checks. Pop 407 was genotyped with 60 simple sequence repeat markers (SSR), pop 423 with 80 SSR markers, and pop 437 with 80 SSR markers. Protein and oil concentrations were measured with near-infrared reflectance spectroscopy. Gmendel was used to construct a genetic map for each population and JoinMap was used to create one map for all populations. Composite interval mapping was done using PlabQTL.