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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF BARLEY

Location: Cereal Crops Research

Title: Elisa Analysis for Fusarium in Barley: Application in Field Nurseries

Authors
item Hill, N - UNIVERSITY OF GEORGIA
item Neate, S - NORTH DAKOTA STATE UNIV
item Cooper, B - BUSCH AG RESOURCES INC
item Horsley, R - NORTH DAKOTA STATE UNIV
item Schwarz, P - NORTH DAKOTA STATE UNIV
item Dahleen, Lynn
item Smith, K - UNIVERSITY OF MN
item Dill-Mackey, R - UNIVERSITY OF MN

Submitted to: American Society of Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2006
Publication Date: November 12, 2006
Citation: Hill, N., Neate, S., Cooper, B., Horsley, R., Schwarz, P., Dahleen, L.S., Smith, K., Dill-Mackey, R. 2006. Elisa analysis for fusarium in barley: application in field nurseries. American Society of Agronomy Abstracts. [CD-ROM] ASA, CSSA, SSSA, Madison, WI.

Technical Abstract: Previously we described a system of quantifying Fusarium Head Blight (FHB) in barley by ELISA. ELISA had lower variability (lower CV's) than visual scoring or deoxynivalenol (DON) analyses. Thus we tested ELISA, DON, and visual assessment of FHB in 1) selections from a barley doubled-haploid mapping population grown in two environments and 2) the North American barley scab evaluation nursery grown at four locations. Visual FHB evaluations and DON had genotype x environment interactions typically found in FHB experiments. However, ELISA had no genotype x environment interaction in either study. Correlations between FHB and DON or FHB and ELISA were significant, but low (r'0.5). Correlations between DON and ELISA were also significant but low (r'0.6). Scattergrams of visual vs. ELISA estimates of FHB suggest visual symptomology is not correlated with abundance of Fusarium on the grain. We conducted laboratory experiments to explain how environmental parameters might explain inconsistency in DON and whether those parameters affected ELISA. In addition we tested for abundance of the antigen specific to the monoclonal antibody used in the ELISA analysis. There was a temperature by osmotic potential interactive effect on DON production in laboratory-grown cultures of Fusarium spp. Neither temperature nor osmotic potential had an effect on abundance of antigen in mycelium. Therefore, ELISA is a more robust estimate of FHB than DON.

Last Modified: 8/1/2014
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