Technical Abstract: Objectives: The dietary assessment methods used in the Jackson Heart Study (JHS) with the entire cohort and a subset of the cohort who participated in a diet and physical activity substudy (DPASS) are reported. These methods were employed to validate culturally explicit data collection instruments developed by Delta Nutrition Intervention Research Initiative (NIRI) for assessing dietary intake in an all African-American cohort. Design: A validation/calibration design was employed. A short food frequency questionnaire (FFQ) was used with all JHS participants (N=5302). A long FFQ and four 24-hour diet recalls were used for the subset of DPASS participants (n=449). Outcome Measures: Completion rates and preliminary macronutrient intakes are reported for the cohort and DPASS methods. Results: Participants (5302) in the JHS full cohort completed the short FFQ. Of these, 572 were enrolled in the DPASS. A total of 506 participants completed four 24-hour dietary recalls, and 499 completed all six measures, including the long FFQ. Preliminary analysis of macronutrients from all three dietary methods supports the validation of the Delta FFQ among Southern African Americans. Validation of dietary instruments in African Americans will help assure that valid conclusions can be drawn regarding the effects of diet on cardiovascular disease in this population.