|Doumit, Matthew - MICHIGAN STATE UNIVERSITY|
|Matukumalli, Lakshmi - GEORGE MASON UNIVERSITY|
|Coutinho, Luiz - UNIVERSITY OF SAO PAULO|
Submitted to: International Conference on Animal Genetics
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 2006
Publication Date: August 10, 2006
Citation: Wiedmann, R.T., Doumit, M.E., Matukumalli, L.K., Sonstegard, T.S., Coutinho, L.L., Nonneman, D.J., Smith, T.P. 2006. Expression profiles of micro-RNAs in swine muscle development. (Abstract) International Conference on Animal Genetics. p. 70. Abstract #C278. Technical Abstract: MicroRNAs (miRs) are small ~18-22 nucleotide-long non-coding RNAs that have been shown to control gene expression by inhibiting translation or targeting messenger RNA for degradation. MiRs have been implicated in control of development, tissue homeostasis, and immune response. We examined the expression profile of porcine miRs present in neonatal biceps femoris using a digital approach of direct sequencing cloned miRs and counting the number of clones for each unique sequence observed. A total of 3,456 clones were sequenced, yielding 3,700 tag sequences representing 600 unique putative miRs. Comparison of unique tags to miRbase identified 241 matches to known miRs, providing evidence that we had successfully cloned porcine miRs. Experiments to determine potential function of these miRs were initiated by comparing the expression profile of neonatal muscle to that derived from cultured porcine satellite cells (adult myoblasts). We sequenced 4,608 clones from a satellite cell miR library, yielding 5,374 tag sequences representing 1,557 unique putative miR sequences that included 339 tags matching annotated miRs in miRbase. Comparison of neonatal muscle and satellite cell miR expression revealed a substantially different miR profile, providing the first data to indicate potential roles of specific miRs in porcine muscle growth and differentiation. The differential expression data identifies targets for siRNA-directed interference with porcine myoblast proliferation and differentiation to probe the role of miRs in muscle development in swine.