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United States Department of Agriculture

Agricultural Research Service

Title: Qtl for the Resistance to Wheat Fusarium Head Blight and Deoxynivalenol Accumulation in Wangshuibai under Field Conditions

Authors
item Ma, H-X - JAAS
item Zhang, K M - JAAS
item Gao, L - JAAS
item Bai, Guihua
item Chen, H G - JAAS
item Cai, Z X - JAAS
item Lu, W Z - JAAS

Submitted to: Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 13, 2006
Publication Date: August 1, 2006
Citation: Ma, H., Zhang, K., Gao, L., Bai, G., Chen, H., Cai, Z., Lu, W. 2006. Qtl for the resistance to wheat fusarium head blight and deoxynivalenol accumulation in wangshuibai under field conditions. Plant Pathology. DOI:10.1111/J.1365-3059.2006.01447.x

Interpretive Summary: Fusarium head blight (FHB), also called wheat scab, significantly reduces wheat yield and quality. Wheat grain infected by the pathogen F. graminearum contains a toxin called DON that affects livestock and human health after consumption. Wangshuibai is a Chinese landrace with good resistance to FHB and DON accumulation in infected grain. An F7 population of recombinant inbred lines (RILs) was derived from the cross between Wangshuibai and Annong 8455. The population was analyzed with molecular markers and evaluated for FHB resistance and DON content in field disease nurseries. We identified two and three genes for low FHB severity and low DON content, respectively. Two genes for low FHB severity were located on chromosome 3B and 2A and three genes for low DON content were located on chromosomes 5A, 2A and 3B. Several molecular markers were identified to link to these genes and should be useful for marker-assisted selection (MAS) of the genes for less FHB and low DON content from Wangshuibai.

Technical Abstract: Wheat Fusarium head blight (FHB) may cause serious losses in grain yield and quality. Production of deoxynivalenol (DON) by Fusarium graminearum in infected grain is detrimental to livestock and is also a safety concern in human foods. Cultivation of cultivars with resistance to FHB and DON accumulation is the most effective strategy for disease control. Wangshuibai is a Chinese landrace with a high level of resistance to FHB and DON accumulation, and an F7 population of recombinant inbred lines (RILs) derived from the cross between Wangshuibai and Annong 8455 was developed for molecular mapping of quantitative trait loci (QTL) for FHB resistance. Proportion of scabbed spiklets (PSS) and DON content were assessed under the field conditions. Composite interval mapping (CIM) revealed that two and three QTL were significantly associated with low PSS and low DON content, respectively, over two years. QTL on chromosome 3B and 2A explained 17% and 11.5% of the phenotypic variance for low PSS, respectively, whereas QTL on chromosome 5A, 2A and 3B explained 12.4, 8.5 and 6.2% of the phenotypic variance for low DON content, respectively. The 3B QTL appeared to be associated mainly with low PSS, and the 5A QTL primarily with low DON content in Wangshuibai. The 2A QTL had minor effect to both low PSS and DON content. The SSR markers, linked to these QTL should be useful for marker-assisted selection (MAS) of QTL for low PSS and low DON content from Wangshuibai.

Last Modified: 10/22/2014
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