|Al-Saleh, Mohammed - SAUDI ARABIA|
Submitted to: Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: August 15, 2006
Publication Date: November 19, 2006
Citation: Al-Saleh, M.A., Chenault, K.D. 2006. Characterization of tomato spotted wilt virus isolates infecting peanut in Southwestern states of USA [abstract]. In: Proceedings of the 9th Arab Congress of Plant Protection, November 19-23, 2006, Damascus, Syria. p. E-89. Available: http://asplantprotection.org/Test/PDF/9thACPP/08_9thACPP.pdf. Technical Abstract: Tomato spotted wilt virus (TSWV) is among the greatest yield-reducing viruses affecting peanut (Arachis hypogaea L.) in all peanut-producing states in U.S. Due to the lack of molecular information on TSWV-isolates associated with peanut in southwestern states, the aim of this study was directed at exploring the diversity of TSWV-isolates in these states. Symptomatic peanut samples were collected from peanut fields and the viral RNA extracted. Complementary DNA (cDNA) was generated by reverse transcriptase PCR (RT-PCR), nonstructural NSm and nucleocapsid N genes were amplified by PCR, then cloned, and sequenced in order to determine their phylogenetic relations. The Clustal W program was used for sequence comparisons, which also included sequences previously reported from other TSWV isolates. Twelve NSm and Five N gene proteins of TSWV infecting peanut in southwest growing areas were sequenced. Several conserved motifs were observed in the amino acid sequences of NSm proteins. Based on nucleotide and amino acid sequences of NSm and N gene proteins, phylogenetic analyses revealed that TSWV-isolates from the southwestern U.S. formed clusters that were distinct from other TSWV-isolates reported previously.