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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT OF DETECTION TECHNOLOGIES FOR TOXINS AND THEIR VALIDATION IN FOOD MATRICES Title: Development of a Sensitive Magnetic Particle Immunoassay for Permethrin

Authors
item Fernando, Rubio - ABRAXIS LLC
item Parrotta, Carmen - ABRAXIS LLC
item Kamp, Lisa - ABRAXIS LLC
item Church, Jennifer - ABRAXIS LLC
item Stanker, Larry

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 31, 2006
Publication Date: June 21, 2006
Citation: Fernando, R., Parrotta, C.D., Kamp, L., Church, J., Stanker, L.H. 2006. Development of a sensitive magnetic particle immunoassay for permethrin. [Abstract]. ACS National Meeting and Exposition. Poster Number AGRO 153.

Technical Abstract: Permethrin is the most widely used insecticide among the pyrethroid family. It’s used in agriculture, home pest control, and in public health programs. Worldwide, the dominant use is on cotton. In the U.S., it’s used on corn, wheat, and alfalfa. Permethrin has been found in streams and rivers and on produce, eg., spinach, tomatoes, celery, lettuce, and peaches. Concerns about environmental contamination have led to the need for more rapid, sensitive, and selective methods of analysis. Enzyme immunoassays (ELISAs) have proven to be rapid, sensitive, accurate, and cost-effective methods. This paper describes the development and performance of a Magnetic Particle-based ELISA for permethrin detection in water and soil samples. The assay exhibits a sensitivity of 0.5 ppb in water with an analytical range of 0.75-15 ppb. Average recoveries in water were 98% with a between and within assay precision of less-than 10.5%. Data obtained with soils as well as comparison to instrumental analysis also will be presented.

Last Modified: 10/1/2014