|Kaiser, Pete - INSTITUTE ANIMAL HEALTH|
|Pevzner, Igal - COBB-VANTRESS, INC|
Submitted to: Avian Immunology Research Group Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 2006
Publication Date: October 21, 2006
Citation: Swaggerty, C.L., He, H., Genovese, K.J., Kaiser, P., Pevzner, I.Y., Kogut, M.H. 2006. The feathering gene is linked to degranulation and oxidative burst not cytokine/chemokine expression or Salmonella enteritidis organ invasion in broilers [abstract]. In: Proceedings of 09th Avian Immunology Research Group Meeting, October 21-24, 2006, Paris, France. p. 58. Technical Abstract: Recently, we showed differences in in vitro heterophil function between parental broilers (Line A>B) and F1 reciprocal crosses (Line C<D) and showed the presence of the fast feathering gene (k) enhanced heterophil functional efficiency while the slow feathering gene (K) reduced efficiency. Based on earlier studies, we want to identify the role of the feathering gene in the innate immune response of broilers. Heterophils were isolated from two-day-old Line C and D chickens separated into males (CM and DM) and females (CF and DF). With respect to the feathering gene, CM=DM (K/k+) whereas CF and DF are K/- and k+/-, respectively. Therefore, if in vitro heterophil function is linked to the feathering gene; heterophils from DF would be functionally efficient compared to heterophils from CF while CM and DM would be equal. Heterophil functions of degranulation and oxidative burst were measured and DF were more (p<0.05) efficient than CF, while the males were comparable indicating the feathering gene is linked to these two functions. Additionally, interleukin (IL)-6 and CXCLi2 mRNA expression were quantitated by real-time quantitative RT-PCR and there were no differences between the groups. Lastly, two-day-old chickens were administered SE and liver/spleen invasion evaluated and the differences are not attributed to the feathering gene. In conclusion, the data indicate the biochemical killing mechanisms of degranulation and oxidative burst are linked to the feathering gene, whereas, cytokine/chemokine mRNA expression and SE organ invasion are not.