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United States Department of Agriculture

Agricultural Research Service

Title: Isolation, Characterization and Cross-Species Amplification of Microsatellite Loci in the Cycad Genus Dioon (Zamiaceae). Potential Utilization in Population Genetics Studies of Dioon Edule

item Meerow, Alan
item Francisco-Ortega, Javier - FLORIDA INTERNATIONAL UNI

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 15, 2006
Publication Date: December 26, 2006
Citation: Moynihan, J., Meerow, A.W., Francisco-Ortega, J. 2006. Isolation, characterization and cross-species amplification of microsatellite loci in the cycad genus dioon (zamiaceae). potential utilization in population genetics studies of dioon edule. Molecular Ecology Notes. 7:72-74.

Interpretive Summary: Nineteen microsatellite DNA genetic marker were developed from the Mexican cycad Dioon edule, of which seven showed allele differences within and among populations of the species. These markers will be used for population and conservation genetic studies in this endangered species, which is highly valued as a unique horticultural plant in subtropical climates.

Technical Abstract: Dioon edule Lindl. (Zamiaceae) is an endemic Mexican cycad. Nineteen microsatellite loci were isolated from three enriched genomic libraries of D. edule var. angustifolium (Miq.) Miq., D. tomasellii De Luca, Sabato & Vazq. Torres, and D. caputoi De Luca, Sabato & Vazq. Torres. Seven of these loci showed polymorphisms in D. edule. Levels of polymorphism were assessed using 112 individuals from seven collection sites throughout the geographic range of this species. The number of alleles per locus ranged from two to five and the observed and expected heterozygosities ranged from 0.0 to 0.9375 and 0.0 to 0.6794 respectively.

Last Modified: 3/27/2015
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