Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 18, 2006
Publication Date: June 1, 2006
Citation: Ling, K. 2006. Two variants of Pepino mosaic virus isolated from imported tomato seed from Chile share high levels of sequence identity with the U.S. isolates. Meeting Abstract. Phytopathology 96:S69. Technical Abstract: Pepino mosaic virus (PepMV), a member of the genus Potexvirus, was first described on pepino (Solanum muricatum A.) grown in South America. In recent years PepMV was reported to infect greenhouse-grown tomatoes. European isolates showed high sequence identity (>99%). On the other hand, two US isolates (US1 and US2) are significantly divergence from that of the European PepMV isolates. In this study, two PepMV isolates (designated as Ch1 and Ch2) were obtained from a commercial tomato seed lot produced in Chile. Our sequence analysis showed that the Ch1 and Ch2 genomes are 6,414 nt and 6,412 nt, respectively (excluding poly A tails), and that they have the highest nucleotide similarity with the two US isolates (98.7% between Ch1 and US1, and 90.7% between Ch2 and US2). Similar to US1 and US2, the two Chilean isolates were most divergent from one another (78% nt identity), and were distinct from the five European isolates (78-86% sequence identity). Phylogenetic analyses using different gene products generated three distinct sequence clusters: US1 and Ch1 in group I, US2 and Ch2 in group II, and the European tomato isolates in group III. These data suggest a common origin for the Chilean and US isolates.