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United States Department of Agriculture

Agricultural Research Service

Title: Linkage of 23s and Rrla Single Nucleotide Polymorphisms to Phenotypic Heterogeneity of Salmonella Enteritidis.

Authors
item Guard, Jean
item Morales, Cesar

Submitted to: Salmonella and Salmonellosis International Symposium
Publication Type: Abstract Only
Publication Acceptance Date: March 27, 2006
Publication Date: May 10, 2006
Citation: Bouldin, J.G., Morales, C. 2006. Linkage of 23s and rrla single nucleotide polymorphisms to phenotypic heterogeneity of salmonella enteritidis. Salmonella and Salmonellosis International Symposium. Saint Malo, France, May 10-12, 2006. 29:p.129.

Technical Abstract: ABSTRACT: The egg-contaminating phenotype of Salmonella enterica serotype Enteritidis was linked to OLE_LINK2OLE_LINK1single-nucleotide polymorphisms (SNPs) occurring in cyaA, which encodes adenylate cyclase that produces cAMP and pyrophosphate from ATP. Ribotyping and sequencing indicated that SNPs in cyaA were coinherited with polymorphisms occurring in rrlC and rrlA 23S ribosomal sequences. Phylogenetic analysis of cyaA discriminated between serotypes and within serotype S. Enteritidis. Serotypes Typhimurium, Heidelberg, and Enteritidis produced one, three, and six cyaA allelic variants, respectively, among the set of 56 isolates examined. Sequence analysis of cyaA was more discriminatory for S. Enteritidis than were PFGE, ribotyping, or Rep-PCR; in addition, it linked genotype to the egg-contaminating phenotype. Asparagine702 of CyaA was converted to serine a biofilm-producing strain, which was significantly deficient in its ability to grow on noncatabolite carbohydrate sources and in egg contamination. These data support the concept that evolution of outbreak potential by the Salmonellae can be monitored on farm and in animal populations by analysis of naturally occurring SNPs that impact metabolism.

Last Modified: 7/22/2014
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