ARS | Publication request: CONSTRUCTION OF A HIGH-DENSITY CYTOGENETIC MAP OF MAIZE CHROMOSOME 9 USING SORGHUM BACS AS FISH PROBES

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 20, 2006
Publication Date: March 9, 2006
Citation: Amarillo, I.E., Bass, H.W., Lawrence, C.J. 2006. Construction of a high-density cytogenetic map of maize chromosome 9 using sorghum BACs as FISH probes [abstract]. 48th Annual Maize Genetics Conference Program and Abstracts. p. 72.

Technical Abstract: The overall goal of this project is to produce a cytogenetic map of the maize genome using RFLP marker-selected sorghum BACs as FISH probes (www.cytomaize.org). Here we describe the recent findings from our detailed pachytene FISH mapping studies in which we have examined a large number of loci spanning chromosome 9. Maize marker-selected sorghum BACs have been chosen for use with the multi-wavelength direct-labeled FISH method described by Koumbaris and Bass (2003, Plant J 35:647). Following collection and deconvolution of the 4-color 3-D images, the chromosome fiber paths were computationally straightened and the FISH signals were mapped using the relative arm position coordinate system that now we refer to as the centiMcClintock map unit (cMC). We have also mapped centromere-linked markers, allowing us to more precisely define the centromere location within linkage map. This project provides unique data that integrates information from a variety of gene maps of maize and sorghum. These results will provide valuable new information for current research on structural genomics, positional cloning, and comparative genomics among the grasses. The cytogenetic FISH map data and chromosome images are available online at http://www.maizegdb.org/cgi-bin/displaymaprecord.cgi?id=892372 through the MaizeGDB.