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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR IDENTIFICATION AND PHYLOGENETIC ANALYSES OF UNIQUE MICROORGANISMS FROM SWINE FECES AND MANURE STORAGE PITS

Location: Bioenergy Research Unit

Title: Swine Feces and Stored Manure: the Search for the Good, the Bad, and the Not So Bad

Authors
item Lawson, Paul - UNIV OKLAHOMA
item Whitehead, Terence
item Cotta, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 28, 2006
Publication Date: July 28, 2006
Citation: Lawson, P.A., Whitehead, T.R., Cotta, M.A. 2006. Swine feces and stored manure: the search for the good, the bad, and the not so bad [abstract]. Congress of the Anaerobe Society of the Americas. p. 49.

Technical Abstract: Storage of swine manure is associated with the microbiological production of a variety of odorous compounds including ammonia, organic acids and alcohols, and sulfides. These compounds can contribute to health problems for swine facility workers and animals alike. In addition, local human populations may also be affected by odors, toxic waste products entering groundwater systems, and these ecosystems acting as reservoirs for Zoonotic pathogens. Determining the microbial populations in swine feces and stored manure that may be responsible for producing the aforementioned compounds or harboring pathogens is a first step in developing strategies to manage/reduce the numbers of these problematic microorganisms. Sequencing of 16S rDNA gene clones derived from culture-independent studies as well as 16S rRNA genes amplified from pure cultures were used by our laboratories to identify the bacterial populations. Both ecosystems were found to contain primarily low (%G+C), Gram positive anaerobic bacteria and have proven to be rich reservoirs of previously unidentified bacteria. Using these techniques, bacterial isolates have been recovered and phylogenetically placed in a number of clostridial rRNA gene clusters and other taxonomic groups. Information obtained during this project has enabled us to recover organisms with sequences corresponding to those found in culture-independent molecular inventories and not represented by extant strains. Using the data obtained, a number of isolates have been described and allocated to novel taxa at the level of both species and genera.

Last Modified: 7/31/2014
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