Submitted to: Congress International Society Develop Comparative Immunology
Publication Type: Abstract Only
Publication Acceptance Date: March 3, 2006
Publication Date: July 1, 2006
Citation: He, H., Genovese, K.J., Kogut, M.H. 2006. Interaction of bacterial CpG-ODN and viral double-stranded RNA (poly I:C) on nitric oxide synthesis and cytokine gene expression in chicken monocytes [abstract]. 10th International Congress of Developmental and Comparative Immunology. p. 122. Technical Abstract: Toll-like receptors (TLRs) recognize microbial components and initiate innate immune responses that control microbial infections. We have investigated the innate immune response of chicken monocytes to bacterial CpG-motif containing oligodeoxydinucleotide, CpG-ODN, and the analog of viral double-stranded RNA, poly I:C, by measuring the induction of NO synthesis and cytokine gene expressions. Our results show ligands poly I:C and CpG-ODN of the TLR3 and TLR9, respectively, synergized the induction of NO in chicken monocytes. When stimulated separately, CpG-ODN induced certain quantities of NO in the chicken monocytes; whereas, poly I:C stimulated very little NO production in the chicken monocytes. In combination, CpG-ODN and poly I:C synergize the effect on NO synthesis, inducing significantly higher level of NO production in chicken monocytes. This synergistic effect of CpG ODN-ODN and poly I:C was also observed on expressions of cytokine mRNA such as IL-1beta and IL-12. In addition, IL-18 expression was differential induced by poly I:C, but not by CpG-ODN. Those results demonstrated differential roles of TLR3 and TLR9 in signaling immune responses to bacterial and viral infections as well as a synergistic interaction between the two signaling pathways.