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United States Department of Agriculture

Agricultural Research Service

Title: Prevalence and Antibiotic Resistance of Salmonella Isolates Recovered from Finishing Pig Herds and Slaughter Facilities in Southern Brazil

Authors
item Kich, Jalusa Deon - EMBRAPA
item Coldebella, Arlei - EMBRAPA
item Mores, Nelson - EMBRAPA
item Fratamico, Pina
item Call, Jeffrey
item Luchansky, John
item Cray, Paula

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 15, 2006
Publication Date: August 13, 2006
Citation: Kich, J., Coldebella, A., Mores, N., Fratamico, P.M., Call, J.E., Luchansky, J.B., Cray, P.J. 2006. Prevalence and antibiotic resistance of salmonella isolates recovered from finishing pig herds and slaughter facilities in southern brazil. {Abstract}. International Association of Food Protection. P2-10. p. 117.

Technical Abstract: The objective of this investigation was to determine the distribution and types of Salmonella in 12 swine finishing herds and a slaughter facility in Santa Catarina, Brazil during 2004. A total of 1,258 samples were colleted and pre-enriched in buffered peptone water, after which, each sample was divided into two portions and enriched in both Tetrathionate and Rappaport-Vassiliadis broth. Following enrichment, a portion from each broth was plated onto Brilliant-Green and XLT4 selective agars. As appropriate, one to four isolates were selected, biochemically confirmed, and were serotyped. From 487 positive samples, a total of 1,255 isolates were recovered and confirmed as Salmonella. The distribution of positive samples were as follows: finishing pen floors 26% (16/61); feed 29% (42/143); feces 44% (52/119); pooled feces 59% (35/59); slaughter holding pens 90% (36/40); lymph nodes 46% (221/478); pre-chilled carcass surfaces 24% (24/98); and post-chilled carcass surfaces 24% (62/260). The most prevalent serotypes were Typhimurium, Panama, Senftenberg, Derby, and Mbandaka. The antibiotic resistance profiles against 15 antibiotics were determined for 582 of the isolates. Results demonstrated that there were 62 different profiles among 582 isolates, 17% (98/582) of isolates were susceptible to all 15 antibiotics tested, 83% (484/582) were resistant to at least one antibiotic, and 43% (248/582) of the isolates were resistant to four or more (multi-resistant). The antibiotics to which resistance was observed most often were: tetracycline 79% (459/582); ampicillin 46% (266/582); kanamycin 40% (234/582); gentamicin 38% (221/582); streptomycin 35% (206/582); and sulfamethoxazole/sulfizoxazole 24% (138/582). Results of this study demonstrate that Salmonella, including multi-antibiotic-resistant strains, are distributed widely over the swine production and slaughter system in Brazil. Furthermore, these findings are important for the identification of risk factors for Salmonella carriage in swine and for the identification of Salmonella control points in the pork production chain.

Last Modified: 9/23/2014