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United States Department of Agriculture

Agricultural Research Service

Research Project: NEW MICROBIAL SYSTEMS FOR UTILIZATION OF GLYCEROL AND PLANT LIPIDS Title: Development of Optimal Culture Conditions for the Production of Extracellular Lipase from Yarrowia Lipolytica Y-2178

Authors
item Lee, G - KYUNGPOOK NATL UNIV, KOR
item Seo, M - KYUNGPOOK NATL UNIV, KOR
item Kim, Y - KYUNGPOOK NATL UNIV, KOR
item Kim, I - KOREA UNIV, KOREA
item Hou, Ching
item Kim, H - KYUNGPOOK NATL UNIV, KOR

Submitted to: American Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 3, 2006
Publication Date: May 3, 2006
Citation: Lee, G.H., Seo, M.J., Kim, Y.J., Kim, I.H., Hou, C.T., Kim, H.R. 2006. Development of optimal culture conditions for the production of extracellular lipase from Yarrowia lipolytica Y-2178. American Oil Chemists' Society Meeting. Bio-2, #6.

Technical Abstract: Lipases are unique enzymes that catalyze the hydrolysis of the ester bonds of long-chain acylglycerol into glycerol and fatty acids at the water-lipid interface and have the ability to generate ester compounds in microaqueous conditions. As such, lipases may be used as catalysts in various industrial fields including medicines, clinical reagents, detergents, food additives, surfactants, cosmetics, and food additives etc. In the last decade, interest in lipases has increased markedly and considerable information is already available about factors affecting the production of lipases. However, there are still needs for new microbial lipases and optimization of their production. Yarrowia lipolytica, NRRL Y-2178, is known as one of the lipase-producing yeast strains with high yield. Increased productivity of lipase without losing its properties is a key factor for low-cost lipase production and new industrial applications. In this study, we report optimized culture conditions for the production of extracellular lipase by Yarrowia lipolytica, NRRL Y-2178. Most of the necessary culture conditions, including carbon and nitrogen sources, were tested for the optimal production of lipase from this strain.

Last Modified: 4/23/2014
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