NEW MICROBIAL SYSTEMS FOR UTILIZATION OF GLYCEROL AND PLANT LIPIDS
Location: National Center for Agricultural Utilization Research
Title: PRODUCTION OF POLYHYDROXY FATTY ACIDES FROM PALMITOLEIC ACID BY PSEUDOMONAS AERUGINOSA PR3
| Kim, H - KYUNGPOOK NATL UNIV, KOR |
| Kim, Y - KYUNGPOOK NATL UNIV, KOR |
| Kim, D - KYUNGPOOK NATL UNIV, KOR |
| Bae, J - DAEGU UNIV, KOREA |
| Kang, S - DAEGU UNIV, KOREA |
| Lee, I - KYUNGPOOK NATL UNIV, KOR |
Submitted to: American Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 3, 2006
Publication Date: May 3, 2006
Citation: Kim, H.R., Kim, Y.M., Kim, D.S., Bae, J.H., Kang, S.C., Lee, I.J., Hou, C.T. 2006. Production of polyhydroxy fatty acids from palmitoleic acid by Pseudomonas aeruginosa PR3. American Oil Chemists' Society. Bio-2, #4.
Hydroxy fatty acids are those which contain hydroxyl groups on fatty acid backbone. They have gained important attention because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. Previously it was reported that a novel bacterial strain Pseudomonas aeruginosa PR3 converted oleic acid to mono- and/or dihydroxy octadecenoic acid. Oleic acid is a 18-carbon monoenoic acid with one cis double bond on carbon 9. This prompted us to address the possibility of utilization of palmitoleic acid as substrate for its hydroxylation by Pseudomonas aeruginosa PR3. Palmitoleic acid has similar structure as oleic acid except for two-carbon short chain length. In this study we report that PR3 can produce a novel dihydroxy fatty acid with 16 carbon chain length (7,10-dihydroxy-8-hexadecenoic acid, DHD) from palmitoleic acid. Optimal culture conditions including carbon and nitrogen sources for the production of DHD from palmitoleic acid by PR3 are also presented.