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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #192476

Title: A NEW ILARVIRUS FOUND IN ROSE

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item GERGERICH, R - UNIVERSITY OF ARKANSAS
item Martin, Robert

Submitted to: Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2006
Publication Date: 5/1/2006
Citation: Tzanetakis, I.E., Gergerich, R.C., Martin, R.R. 2006. A new ilarvirus found in rose. Plant Pathology. 55:568.

Interpretive Summary: Rose rosette is one of a group of poorly understood diseases where the causal agent is transmitted by eriophyid mites. In an effort to identify the causal agent of rose rosette disease, double stranded RNA was extracted from several symptomatic rose plants. One plant gave a dsRNA banding patter similar to that of ilarviruses, which was not surprising since several viruses in this genus are known to infect rose. The dsRNA was cloned and sequenced and the sequence compared to previously published sequences of plant viruses. The seqeunce obtained was approximately 70% identical to that of Tobacco streak virus and less than 50% identical to Prunus necrotic ringspot virus and Apple mosaic virus, three ilarviruses known to infect rose. While this work was being conducted a new virus was described in blackberry that causes a chlorotic ringspot disease. The new virus we isolated from rose is related most closely to the blackberry chlorotic ringspot virus and is considered a strain of that virus. This virus is not responsible for the rosette disease of rose.

Technical Abstract: Rose rosette, a devastating disease in the midwestern and southeastern United States, is vectored by the eriophyoid mite Phyllocoptes fructiphilus and the causal agent is believed to be a virus. In an attempt to characterize the causal agent of the disease, double stranded RNA (dsRNA) was isolated from diseased plants. One of the plants assayed gave dsRNA band pattern similar to that of viruses in the Bromoviridae family. DsRNA was cloned as described and several clones were sequenced to determine the origin of the bands. Sequence analysis indicated that a new virus can infect rose. The novel virus, named hereafter Rose virus 1 (RV1) shared about 70% nucleotide identities with Strawberry necrotic shock virus (SNSV) and Tobacco streak virus (TSV) and less than 50% identities with the other two rose infecting ilarviruses, Prunus necrotic ringspot virus and Apple mosaic virus. Regions of RV1 RNA 1 and 3 have been deposited in GenBank under accession numbers DQ329377 and DQ329378 respectively.