Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Bioconversion of N-6 and N-3 Pufas by Bacillus Megaterium Ala2

Author
item Hou, Ching

Submitted to: International Society for the Study of Fatty Acids and Lipids
Publication Type: Abstract Only
Publication Acceptance Date: July 28, 2006
Publication Date: July 28, 2006
Citation: Hou, C.T. 2006. Bioconversion of N-6 and N-3 pufas by Bacillus megaterium ALA2 [abstract]. International Society for the Study of Fatty Acids and Lipids. S-29. p. 43.

Technical Abstract: We have been working on producing value-added products from vegetable oils by biocatalysis including healthy oils and new oxygenated fatty acids. Oxygenated fatty acids can be used as specialty chemicals and biomedicals. Bacillus megaterium ALA2 (NRRL B-21660) converted n-6 and n-3 PUFAs to value-added oxygenated products. Linoleic acid was converted to: trihydroxy-9(S)-octadecenoic acid (THOA), diepoxy-bicyclic unsaturated fatty acid (DEOA), and tetrahydrofuranyl unsaturated fatty acid (TFOA) with 12,13,17-THOA as the main product. The linoleic acid bioconversion pathway by strain ALA2 was established. Strain ALA2 also converted EPA, DHA, arachironic acid and '-linolenic acid to new oxygenated products including DEOAs, TFOAs, and THOAs. Recently, we found that strain ALA2 possesses a monooxygenase system oxidizing palmitic acid to omega-1, omega-2, and omega-3 hydroxy palmitic acids similar to those reported in the well-studied catalytically self-sufficient P450 monooxygenase of Bacillus megaterium ATCC14581 and B. subtilis. We also found that strain ALA2 produced 9,10-epoxy-12-(Z)-octadecenoic acid from linoleic acid. These findings enable us to compare and utilize known Bacillus gene data with our strain ALA2 for metabolic pathway engineering to produce products of interest.

Last Modified: 10/21/2014
Footer Content Back to Top of Page