Author
HALEGREN, A - OREGON STATE UNIVERSITY | |
TZANETAKIS, I - OREGON STATE UNIVERSITY | |
Martin, Robert |
Submitted to: Acta Horticulturae
Publication Type: Abstract Only Publication Acceptance Date: 3/21/2006 Publication Date: 5/1/2006 Citation: Halegren, A.B., Tzanetakis, I.E., Martin, R.R. 2006. Characterization of an aphid-transmitted virus associated with black raspberry decline in Oregon. Acta Horticulturae. Interpretive Summary: Technical Abstract: At least one virus was consistently associated with declining black raspberries—Black raspberry decline associated virus (BRDaV). Grafting and aphid transmissions do not induce the characteristic tip necrosis characteristic of Black raspberry necrosis virus (BRNV), a previously described but poorly characterized virus of black raspberry. The virus has been aphid transmitted successfully to virus-free black raspberry, as well as to Nicotiana tabacum and N. benthamiana. Though difficult, mechanical transmission to N. benthamiana was successful. DsRNA extraction from declining black raspberry revealed two major bands of about 8 and 9 kb, suggesting either infection with two viruses or a virus that contains two pieces of nucleic acid. The virus was cloned and sequenced, and an RT-PCR detection method was developed that allowed for its rapid detection in several symptomless hosts, including R. idaeus (red raspberry), R. laciniatus (Evergreen blackberry), R. armeniacus (Himalaya blackberry), and ‘Marion’ blackberry, as well as detection in multiple cultivars of black raspberry. The complete genome of BRDaV was obtained recently, and it is related most closely to the Sadwavirus genus of the family Sequiviridae, sharing closest homology to Strawberry mottle virus. Interestingly, BRDaV contains a 566 bp insert at the very end of the RNA-dependent-RNA-polymerase with homology to viruses of the Flexiviridae. This insert likely was acquired from a transposition event with a co-infecting virus. |