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ARS Home » Pacific West Area » Corvallis, Oregon » Research » Publications at this Location » Publication #192021

Title: COMPLETE NUCLEOTIDE SEQUENCE AND EPIDEMIOLOGY OF RASPBERRY MOTTLE ASSOCIATED VIRUS

Author
item TZANETAKIS, I - 2072-00-00
item HALGREN, A - 2072-00-00
item Martin, Robert

Submitted to: Acta Horticulturae
Publication Type: Abstract Only
Publication Acceptance Date: 3/8/2006
Publication Date: 5/1/2006
Citation: Tzanetakis, I.E., Halgren, A.B., Martin, R.R. 2006. Complete nucleotide sequence and epidemiology of raspberry mottle associated virus. Acta Horticulturae.

Interpretive Summary:

Technical Abstract: During routine graft indexing of Rubus clones at the USDA-ARS Horticultural Crops Research Laboratory in Corvallis, Oregon, a ‘Glen Clova’ plant that originated in Scotland exhibited severe symptoms, including stunting and apical necrosis on ‘Munger’ black raspberry. Cloning of viral dsRNA obtained from the plant and sequence analysis indicated the presence of a novel virus, a member of the Closteroviridae, and possibly additional viruses. Detection protocols developed against the virus were used and identified the virus in breeding and field material from Washington State as well as several additional raspberry clones from Scotland. Graft indexing of one of those plants, free of other known raspberry viruses, gave mottling symptoms on indicators and the virus was thus temporarily designated as Raspberry mottle associated virus (RMaV). The complete nucleotide sequence of the virus has been obtained and exceeds 17 kilobases. Phylogenetic analysis indicated that RMaV is closely related to members of the Closterovirus genus, the aphid-borne closteroviruses. The genome organization is an intermediate of Citrus tristeza virus and Beet yellows virus with two putative leader proteases and a conserved array of open reading frames at the 3’ proximal terminus. Currently, additional tests are in progress to examine the possibility that RMaV is the causal agent of one of the graft-transmittable diseases previously identified in Rubus.