Technical Abstract: Physicochemical properties of acorn (Quercus palustris) starch were studied. Acorn starch granules were spherical or ovoid, with diameters ranging from 3-17 micrometers. Acorn starch exhibited A-type X-ray diffraction pattern, an apparent amylose content of 43.4% and absolute amylose content of 25.9%. Relative to other A-type starches, acorn amylopectin had comparable weight-average molecular weight (3.9 x 108), gyration radius (288 nm), and density (16.3 g/mol/nm3). Average amylopectin branch chain-length was DP 25.5. Onset gelatinization temperature was 65.0 deg C, and peak gelatinization temperature was considerably higher (73.7 deg C). Enthalpy change of gelatinization was very high compared to non-mutant starches (20.8 J/g). Amylose-lipid thermal transition was not observed. Starch retrograded for 7 d at 4 deg C had very high peak melting temperature (54.2 deg C) relative to other A-type starches. Final (260 RVU) and setback (138 RVU) viscosity of 8% acorn starch paste was high relative to other starches, and the pasting temperature was 71.5 deg C.