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United States Department of Agriculture

Agricultural Research Service

Title: Introgression of a Tombusvirus Resistance Locus from N. Edwardsonii Var. Columbia to N. Clevelandii

Authors
item Schoelz, James - UNIV.MISSOURI,COLUMBIA
item Wiggins, B. - UNIV.MISSOURI,COLUMBIA
item Wintermantel, William
item Ross, Kathleen

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 28, 2005
Publication Date: May 1, 2006
Citation: Schoelz, J.E., Wiggins, B.E., Wintermantel, W.M., Ross, K. 2006. Introgression of a tombusvirus resistance locus from n. edwardsonii var. columbia to n. clevelandii. Phytopathology. 96:453-459. DOI:10.1094/PHYTO-96-0453

Technical Abstract: A new variety of Nicotiana, N. edwardsonii var. Columbia, was evaluated for its capacity to serve as a new source for virus resistance genes. Columbia was developed from a hybridization between N. glutinosa and N. clevelandii, the same parents used for the formation of the original N. edwardsonii. However in contrast to the original N. edwardsonii, crosses between Columbia and either of its parents are fertile. Thus, the inheritance of virus resistance genes present in N. glutinosa could be characterized by using Columbia as a bridge plant in crosses with the susceptible parent, N. clevelandii. To determine how virus resistance genes would segregate in interspecific crosses between Columbia and N. clevelandii, we followed the fate of the N gene, a single dominant gene that specifies resistance to Tobacco mosaic virus (TMV). Our genetic evidence indicated that the entire chromosome containing the N gene was introgressed into N. clevelandii to create and addition line, designated N. clevelandii Line 19. Although Line 19 was homozygous for resistance to TMV, it remained susceptible to Tomato bushy stunt virus (TBSV) and Cauliflower mosaic virus (CaMV) strain W260, indicating that resistance to these viruses must reside on other N. glutinosa chromosomes. We also developed a second addition line, N. clevelandii Line 36, which was homozygous for resistance to TBSV. Line 36 was susceptible to TMV and CaMV strain W260, but was resistant to other tombusviruses, including Cucumber necrosis virus, Cymbidium ringspot virus, Lettuce necrotic stunt virus, and Carnation Italian ringspot virus.

Last Modified: 10/1/2014